• Skip to main content
  • Skip to primary sidebar

KXTbio

Powered by Proteins

  • Home
  • Products
    • Proteins
      • GTPases
      • PLCs
    • Biosensors
  • Services
    • High-throughput screening
    • Customized proteins
  • Science
    • Resources
    • Proprietary PLC Crystal Structure
    • Publications
  • Company
    • About us
    • Careers
    • Licensing
    • Contact
Products > Proteins > GTPases > Rac1 C-terminal His6-tagged

Rac1 C-terminal His6-tagged

$200 – $800

Clear
SKU: N/A Category: GTPases

  • Additional information

Product information

Member of the Rho family of GTPases1 within the superfamily of Ras-like GTPases. Similar to most GTPases, Rac1 cycles between GDP- and GTP-bound states; the GTP-bound form engages a set of diverse effectors to regulate cell processes. In particular, Rac1 promotes lamellipodia and membrane ruffles necessary for phagocytosis and cell migration. Nucleotide cycling is regulated by guanine nucleotide exchange factors (GEFs), guanine dissociation inhibitors (GDIs) and GTPase activating proteins (GAPs).

Protein is > 99% pure as determined by densitometry of the final sample submitted to SDS-PAGE and stained with Coomassie blue (Fig. 1). Rac1 was heterologously expressed in E. coli as a histidine-tagged protein and purified by a combination of affinity, ion exchange, and gel exclusion chromatographies. The histidine-tagged was not removed leaving a (His)6 peptide at the C-terminus after residues 1-189 of full-length, human Rac1. The terminal cysteine, which would normally be post-translationally isoprenylated in eukaryotic cells, has been mutated to serine (C189S).

  1. Hodge RG, et al., Regulating Rho GTPases and their regulators. Nat Rev Mol Cell Biol 17, 496-510 (2016).

Properties:

Species: Homo sapiens.
Sequence: Residues 1-189 of Rac1. The terminal cysteine is mutated to serine (C189S) and the C-terminus retains an histidine-tag (His6).
Calculated molecular weight: 21,917 g/mol.
Experimental molecular weight: NA.
Extension coefficient: 22,920 M-1cm-1 (assuming all cysteines remain reduced).
Amount: 100 or 500 μg.
Concentration: 1 mg/ml (determined by absorbance at 280 nm).
Buffer: 25 mM Tris pH 7.5, 150 mM NaCl, 5% glycerol and 1mM DTT.
Form: Frozen liquid.

Storage and stability:

Storage: -80 °C. After initial thaw, aliquot and store at -80 °C until further use.
Stability: Stable for up to 12 months at -80 °C and up to 2 days at 4 °C. Repeated freeze-thaw cycles not recommended.
Notes: Please see accompanying sheet describing activity.

Additional information

Weight

100 µg $200, 200 µg $250, 500 µg $800

Related products

  • Cdc42

    $200 – $800
    Select options
  • K-Ras

    $200 – $800
    Select options
  • Arf6 N-terminal His6-tagged $200 – $800 Select options

  • Arf1 N-terminal His6-tagged

    $200 – $800
    Select options

Primary Sidebar

Product literature

Rac1 C-terminal His6-tagged
Product information


Rac1 C-terminal His6-tagged
Quality control information


Related products

  • Arf1 N-terminal His6-tagged

    $200 – $800
    Select options
  • Arf6 N-terminal His6-tagged $200 – $800 Select options

  • Rac1

    $200 – $800
    Select options

KXTbio, Inc.

Email: info@kxtbio.com

Shipping information
Terms and conditions

Please follow us

LinkedIn

KXTbio Newsletter

Want to find out about our latest research and receive regular whitepapers, research articles and news?



Copyright © 2017-18