PLCglow™is a small molecule that directly reports the enzymatic activity of phospholipase C (PLC) isozymes1,2. PLCglow™ is hydrolyzed by PLCs to generate inositol trisphosphate (IP3), quinomethide, and 6-aminoquinoline. Relative to PLCglow™, the aminoquinoline is highly fluorescent (λex/em = 344/530 nm) (Fig. 1).
- Huang et al., A fluorogenic, small molecule reporter for mammalian phospholipase C isozymes. ACS Chemical Biology 6(3), 223-8 (2011).
- Huang et al., Small molecule inhibitors of phospholipase C from a novel high-throughput screen. JBC 288(8), 5840-8 (2013).
Molecular weight: 1127 g/mol as triethylamine salt (824 g/mol for compound alone).
Amount: 50 μg or 200 μg
Storage and stability:
Storage temperature: -80 °C.
Stability: Stable for up to 12 months at -80 °C and up to 14 days at 4 °C in water. PLCglow™ is not stable for long periods in several common buffers and stock solutions should be stored only in water. Repeated freezing and thawing is not recommended.
Notes: Results will vary depending on the sensitivity of the fluorometer and the specific activity of the PLC isozyme. In order to obtain optimal results, we recommend testing a dilution series of WH-15 and PLC isozyme. WH-15 is designed to work with purified proteins and cellular lysates. In contrast, WH-15 will not enter intact cells and is therefore inappropriate for measuring intracellular PLCs.