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Products > Proteins > PLCs > PLCγ1

PLCγ1

$200

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SKU: N/A Category: PLCs
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Product information

PLCγ1 is a calcium-dependent lipase that hydrolyzes phosphatidylinositol 4,5- bisphosphate (PIP2) to produce the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). PLCγ1 is activated upon phosphorylation by a wide variety of receptor tyrosine kinases and soluble tyrosine kinases to control proliferation and differentiation.1,2

Protein is > 94% pure as determined by densitometry of the final sample submitted to SDS-PAGE and stained with Coomassie blue (Fig. 1). PLCγ1 was heterologously expressed in High 5 insect cells as a histidine-tagged protein and purified by a combination of affinity, ion exchange, and gel exclusion chromatographies. The tag was removed prior to final purification leaving a Ser-Gln-Ala tripeptide at the N-terminus followed by residues 1-1219 of rat PLCγ1. Purified PLCγ1 lacks 71 residues from its C-terminus that are not conserved. This truncation improves purity and solubility without affecting lipase activity.

  1. Kadamur G, et al., Mammalian phospholipase C. Annu Rev Physiol 75, 15.1-15.28 (2013).
  2. Gresset A, et al., Mechanism of phosphorylation-induced activation of phospholipase C-γ isozymes. J Biol Chem 285, 35836-47 (2010).

Properties

Species: Rattus norvegicus – 94% identity to Homo sapiens.
Sequence: PLCγ1 (residues 1-1219); lacks 71 residues from its C-terminus that are not conserved. The N-terminus retains a Ser-Gln-Ala tripeptide remaining from the affinity tag.
Calculated molecular weight: 140,469 g/mol.
Experimental molecular weight: NA.
Extension coefficient: 179,010 M-1cm-1 (assuming all cysteines remain reduced).
Amount: 20 μg.
Concentration: 0.2 mg/ml (determined by absorbance at 280 nm).
Buffer: 20 mM Hepes pH 7.5, 150 mM NaCl, 5% glycerol and 1 mM DTT.
Form: Frozen liquid.

Storage and stability

Storage: -80 °C. After initial thaw, aliquot and store at -80 °C until further use.
Stability: Stable for up to 12 months at -80 °C and up to 2 days at 4 °C. Repeated freeze-thaw cycles not recommended.
Notes: Please see accompanying sheet describing activity.

Additional information

Weight

10 µg $150, 20 µg $200, 50 µg $500

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Product literature

PLCγ1 Product information

PLCγ1 Quality control information

Related publications

Fluorescence-based biochemical high throughput primary assay to identify inhibitors of phospholipase C isozymes (PLCγ1).
+ Read abstract
Extracellular stimuli including hormones, growth factors, and neurotransmitters promote activation of phospholipase C (PLC) isozymes and cleavage of the membrane lipid phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2) into the classical second messengers, diacylglycerol and inositol 1,4,5- trisphosphate (IP3) [1]. These second messengers coordinately control numerous signaling cascades through the mobilization of intracellular Ca2+ stores and the activation of protein kinase C. Aberrant regulation of PLCs contribute to diverse human diseases including cancer [2-4], cardiovascular diseases [5-6], and neuropathic pain [7], as well as schizophrenia and epilepsy [5, 8-9]. Consequently, small molecule PLC inhibitors will be valuable pharmacological tools to dissect the roles of PLCs in development and disease, and could potentially serve as candidates for drug development. Read the full article here

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