• Skip to main content
  • Skip to primary sidebar

KXTbio

Powered by Proteins

  • Home
  • Products
    • Proteins
      • GTPases
      • PLCs
    • Biosensors
  • Services
    • High-throughput screening
    • Customized proteins
  • Science
    • Resources
    • Proprietary PLC Crystal Structure
    • Publications
  • Company
    • About us
    • Careers
    • Licensing
    • Contact
Products > Proteins > PLCs > PLCβ1

PLCβ1

$200

Clear
SKU: N/A Category: PLCs
  • Additional information

Product information

PLCβ1 is a calcium-dependent lipase that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). PLCβ1 is mainly expressed in the cerebral cortex and hippocampus, where the enzyme regulates neuronal activity. PLCβ1 is directly activated by Gα subunits related to Gαq and Gβγ heterodimers.1,2

Protein is > 98% pure as determined by densitometry of the final sample submitted to SDS-PAGE and stained with Coomassie blue (Fig. 1). PLCβ1 was heterologously expressed in High 5 insect cells as a histidine-tagged protein and purified by a combination of affinity, ion exchange, and gel exclusion chromatographies. The tag was removed prior to final purification leaving a Gly-Ala-Met-Asp-Pro-Glu-Phe peptide at the N-terminus followed by residues 1-1216 of rat PLCβ1.

  1. Kadamur G, et al., Mammalian phospholipase C. Annu Rev Physiol 75, 15.1-15.28 (2013).
  2. Lyon A.M, et al., Structural Insights into Phospholipase C-β Function. Mol Pharmacol 83, 488-500 (2013).

Properties

Species: Rattus norvegicus – 97% identity to Homo sapiens.
Sequence: Residues 1-1216 of PLCβ1. The N-terminus retains a Gly-Ala-Met-Asp-Pro-Glu-Phe peptide remaining from the affinity tag.
Calculated molecular weight: 139,092 g/mol.
Experimental molecular weight: NA.
Extension coefficient: 92,600 M-1cm-1 (assuming all cysteines remain reduced).
Amount: 20 μg.
Concentration: 0.2 mg/ml (determined by absorbance at 280 nm).
Buffer: 20 mM Hepes pH 7.5, 150 mM NaCl, 5% glycerol and 1 mM DTT.
Form: Frozen liquid.

Storage and stability

Storage: -80°C. After initial thaw, aliquot and store at -80°C until further use.
Stability: Stable for up to 12 months at -80°C and up to 2 days at 4°C. Repeated freeze-thaw cycles not recommended.
Notes: Please see accompanying sheet describing activity.

Additional information

Weight

10 µg $150, 20 µg $200, 50 µg $500

Related products

  • PLCβ2

    $200
    Select options
  • PLCγ1

    $200
    Select options
  • PLCβ3

    $200
    Select options
  • PLCδ1

    $200
    Select options

Primary Sidebar

Product literature

PLCβ1 Product information

PLCβ1 Quality control information

Related products

  • PLCδ1

    $200
    Select options
  • PLCγ2

    $200
    Select options
  • PLCβ2

    $200
    Select options

KXTbio, Inc.

Email: info@kxtbio.com

Shipping information
Terms and conditions

Please follow us

LinkedIn

KXTbio Newsletter

Want to find out about our latest research and receive regular whitepapers, research articles and news?



Copyright © 2017-18