We focus on the development of reagents and facile assays that provide robust, cost-effective readouts of signaling cascades controlled by heterotrimeric G proteins and GTPases.

 

Available screening platfroms:

 

PLCglowPLC screens

PLCglow is a unique reagent explicitly developed to monitor the enzymatic activity of mammalian phospholipase C isozymes using fluorescence. PLCglow works with cell lysates and enables high-throughput screens with purified proteins.

 

GTPaseGTPase screens

We have developed a series of fluorescent assays to monitor the activation of diverse GTPases and heterotrimeric G proteins. The assays have been developed to enable high-throughput screens.