Ni-NTA Agarose, 100ml

1000

 

Product Description

KXTbio Ni-NTA Agarose was developed for the affinity purification of proteins carrying a polyhistidine tag. This affinity chromatography matrix is based on BioWorks Workbeads, consisting of 7.5% cross- linked agarose. The material is highly porous to allow for optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes under low pressure with flow rates up to 6 mL/min (optimal 0.5 – 2 mL/min). Our agarose is very homogeneous in size with a medium particle diameter of 40 μm, yielding a high degree of reproducibility between individual purification runs.

An NTA ligand is coupled to the agarose matrix and carefully loaded with nickel ions to obtain an affinity matrix with highest binding capacity for histidine residues. The metal ion capacity is > 15 μeqv Ni2+/mL. Other possible metal ions are Co2+, Zn2+, Fe3+, and Al3+, resulting in different affinities, e.g. for zinc- finger proteins or phosphorylated proteins. If required, the nickel ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix can be recharged with a different metal ion. Alternatively, please contact us for unloaded NTA agarose matrix.

KXTbio Ni-NTA Agarose is delivered as a 50% (v/v) suspension. The suspension contains 20% ethanol to prevent microbial growth.

Protein Binding Capacity

The protein binding capacity is up to 70 mg/mL, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.

Compatibility

KXTbio Ni-NTA Agarose is very stable and can resist the following conditions in most situations: pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile. 

 

Also avialble in 25 and 50 ml sizes. Please inquire at info@kxtbio.com for pricing. 

USD 899.00

Go back