Ni-NTA Magnetic beads, 25 ml

1002

25 ml

 

Please inquire for other size options and pricing at info@kxtbio.com

 

Product Descrption

KXTbio Ni-NTA MagBeads were developed for the affinity purification of proteins carrying a polyhistidine tag. The affinity matrix is based on spherical magnetic agarose beads, consisting of 6% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. Our magnetic beads are very homogeneous in size with a medium particle diameter of 30 μm, yielding a high degree of reproducibility between individual purification runs.

An NTA ligand is coupled to the agarose and carefully loaded with nickel ions to obtain a matrix with highest binding capacity for histidine residues. The metal ion capacity is > 12 μeqv Ni2+/mL. Other possible metal ions are Co2+, Zn2+, Fe3+, Al3+, and Cu2+, resulting in different affinities, e.g. for zinc-finger proteins or phosphorylated proteins. If required, the nickel ions can be removed from the magnetic beads using 5 wash steps with 100 mM EDTA, and the magnetic beads can be recharged with a different metal ion. Alternatively, please contact us for unloaded KXTbio NTA magnetic beads.

KXTbio Ni-NTA MagBeads are delivered as a 25% suspension. Therefore, 1 mL suspension will yield a 250 μL bed volume. The suspension contains 20% ethanol to prevent microbial growth.

Protein Binding Capacity

The protein binding capacity is 70 mg protein per mL of settled beads, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.

Compatibility

KXTbio Ni-NTA MagBeads are very stable and can resist the following conditions in most situations: pH 2-4, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile. 

USD 1,199.00

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