Glutathione Magnetic beads, 25 ml

3002

Please inquire for other size options and pricing at info@kxtbio.com

Product Description

KXTbio Glutathione MagBeads are developed for the affinity purification of glutathione-S-transferase (GST) fusion proteins. The affinity matrix is based on spherical magnetic agarose beads, consisting of 6% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. Our magnetic beads are very homogeneous in size with a medium particle diameter of 30 μm, yielding a high degree of reproducibility between individual purification runs.

Glutathione is coupled to the magnetic agarose beads to obtain an affinity matrix with highest binding capacity for GST fusion proteins. Because the purification method depends on correctly folded GST protein, only native conditions can be used.

KXTbio Glutathione MagBeads are delivered as a 25% suspension.  The suspension contains 20% ethanol to prevent microbial growth.

Protein Binding Capacity

The protein binding capacity is up to 10 mg/mL settled beads, as determined by purification of glutathione-S-transferase from E.coli cleared lysates, and quantified via spectrophotometry.

Compatibility

KXTbio Glutathione MagBeads are stable in most commonly used aqueous buffers with pH 3 – 12, (e.g. 1 M sodium acetate, pH 4.0, or 6 M guanidine-hydrochloride), organic solvents (e.g., 70% (v/v) ethanol), and 1% (w/v) SDS for 1 h at room temperature. 

USD 899.00

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