Glutathione Agarose, 100 ml

3000

 

Please inquire for other size options and pricing at info@kxtbio.com

 

Product Descrption

KXTbio Glutathione Agarose was developed for the affinity purification of glutathione-S-transferase (GST) fusion proteins. This affinity chromatography matrix is based on BioWorks Workbeads, consisting of 7.5% cross-linked agarose. The material is highly porous to allow for optimal protein interaction. Cross- linked agarose is also physically very stable, making it suitable for purification processes under low pressure with flow rates up to 6 mL/min (optimal 0.25 – 1 mL/min). Our agarose resin is very homogeneous in size with a medium particle diameter of 40 μm, yielding a high degree of reproducibility between individual purification runs.

Glutathione has been coupled to the agarose to obtain an affinity matrix with highest binding capacity for GST fusion proteins. PureCube Glutathione Agarose can be used for batch purification, as well for low pressure column purification, and is compatible with all prokaryotic and eukaryotic expression systems. Because the purification method depends on correctly folded GST protein, only native conditions can be used.

KXTbio Glutathione Agarose is delivered as a 50% suspension. The suspension contains 20% ethanol to prevent microbial growth.

Protein Binding Capacity

The protein binding capacity is up to 10 mg/mL resin, as determined by purification of glutathione-S- transferase from E.coli cleared lysates, and quantified via spectrophotometry.

Compatibility

For cleaning purposes, KXTbio Glutathione Agarose is very stable and can resist the following conditions in most situations:
All commonly used aqueous buffers, from pH 3 – 12, e.g. 1 M sodium acetate, pH 4.0, or 6 M guanidine- hydrochloride, organic solvents (e.g., 70% (v/v) ethanol), 1% (w/v) SDS, 0.1 M NaOH, 0.1 M HCl. 

 

Available in 25 and 50 ml sizes. Please inquire at info@kxtbio.com for pricing. 

USD 1,200.00

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