Co-NTA Agarose, 100 ml

2000

 

Product Descriptionpage1image6376 page1image6536 page1image6960 page1image7120

 

KXTbio Co-NTA Agarose was developed for the affinity purification of proteins carrying a polyhistidine tag. This affinity chromatography matrix is based on BioWorks Workbeads, consisting of 7.5% cross- linked agarose. The material is highly porous to allow for optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes under low pressure with flow rates of up to 6 mL/min (optimal 0.5–2 mL/min). Our agarose is very homogeneous in size with a medium particle diameter of 40 μm, yielding a high degree of reproducibility between individual purification runs.

An NTA ligand is coupled to the agarose matrix and carefully loaded with cobalt ions to obtain an affinity matrix with highest binding capacity for histidine residues. The metal ion capacity is > 15 μeqv Co2+/mL. Other possible metal ions are Ni2+, Zn2+, Fe3+, and Al3+, resulting in different affinities, e.g. for zinc-finger proteins or phosphorylated proteins. If required, the cobalt ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix can be recharged with a different metal ion. Alternatively, please contact us for unloaded NTA agarose matrix.

KXTbio Co-NTA Agarose is delivered as a 50% (v/v) suspension. The suspension contains 20% ethanol to prevent microbial growth.

Protein Binding Capacity

The protein binding capacity is 30 mg/mL resin, as determined by purification of 6xHis-tagged GFP protein from E.coli cleared lysates, and quantified via spectrophotometry.

Compatibility

KXTbio Co-NTA Agarose is very stable and can resist the following conditions in most situations: pH 2-14, 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile. 

 

Also available in 25 and 50 ml sizes. Please inquire at info@kxtbio.com for pricing.

USD 929.00

Go back